Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

Protocol

Cellular lipid uptake with flow cytometry readout

This protocol describes cellular lipid uptake with flow cytometry readout.

Tool

pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A

Transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A.

Article

Rodent models based on endolysosomal genes involved in Parkinson’s disease

Review: This review summarizes parkinsonian phenotypes in rodent models targeting genes that have a role in endolysosomal pathways and future steps to better understand the contribution of endolysosomal dysfunction to PD.

Article

Inter-organellar communication in Parkinson’s and Alzheimer’s disease: looking beyond endoplasmic reticulum-mitochondria contact sites

Review: Here, authors summarize the contributions of membrane contact sites in dysregulation of inter-organellar communication, taking findings from Parkinson’s and Alzheimer’s as major examples.

Article

Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction

Review: The development of the Lyso-IP approach and similar methods now allow for lysosomal purification within ten minutes. This review discusses the impact of this new methodology in uncovering the role of lysosomes in neurodegenerative conditions.

Article

ATP13A2 Regulates Cellular α-Synuclein Multimerization, Membrane Association, and Externalization

Published: ATP13A2 loss-of-function mutations cause lysosomal deficiency and are linked to Parkinson’s disease and alpha-synuclein pathology. The authors found that loss of ATP13A2 disrupts lysosomal membrane integrity and causes alpha-synuclein multimerization. Further, they showed that increased levels of ATP13A2 had a protective effect on alpha-synuclein aggregation.

Tool

pLenti HsATP10B_WT-T2A-His-flag-TMEM30A

Transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A.

Protocol

Lysosome proteolysis analysis with DQ-BSA

Protocol to analyze lysosomal proteolysis in astrocytes generated from iPSCs.

Article

The lysosome as a master regulator of iron metabolism

Review: This review focuses on the role that the lysosome plays in maintaining iron homeostasis and how lysosomal iron dysregulation contributes to disease.

Article

P5B-ATPases in the mammalian polyamine transport system and their role in disease

Review: This review brings together the current knowledge of the cellular function of the mammalian polyamine transport system, focusing on the role of P5B-ATPases ATP13A2-5.

Protocol

Radiolabeled polyamine uptake in cells

This protocol provides a technique to determine the radiolabeled polyamine uptake capacity in cells, via the acquisition of disintegrations per minute (DPM) using a Liquid Scintillation Counter.

 

Protocol

Fluorescently labeled polyamine uptake (via Flow Cytometry)

Assess polyamine uptake capacities of a specific cell line after incubation with fluorescently labeled polyamines and mean fluorescence intensity acquisition via flow cytometry.
Access the interview with Mujahid Azfar, MSc, about this protocol.