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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Tool
pLenti HsATP10B_WT-T2A-His-flag-TMEM30A
Transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A.
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Article
Lysosomal dysfunction in neurodegeneration: emerging concepts and methods
Review: This review summarizes key technological advances that have led to a better understanding of the contribution of the lysosome to neurodegeneration and highlights key questions to be addressed moving forward.
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Tool
pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A
Transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A.
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Article
CRISPR/Cas9-based functional genomics in human induced pluripotent stem cell–derived models: Can “the stars align” for neurodegenerative diseases?
Viewpoint article on CRISPR/Cas9-based functional genomics in human induced pluripotent stem cell–derived models.
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Protocol
Western blotting to detect ATP13A2 and ATP13A3
Protocol to detect ATP13A2 and ATP13A3 via Western blotting.
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Article
Disruption of lysosomal proteolysis in astrocytes facilitates midbrain proteostasis failure in an early-onset PD model
Preprint: Accumulation of advanced glycation end products (AGEs) on biopolymers accompany cellular aging and drives poorly understood disease processes. Here, authors studied how AGEs contribute to development of early on-set Parkinson’s disease (PD) caused by loss-of-function of DJ1, a protein deglycase.
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Protocol
Evaluating endocytic rate in cells
Assess endocytic rate in cells using tagged transferrin (Alexa647) and flow cytometry readout.
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Fluorescently labeled polyamine uptake (via Flow Cytometry)
Assess polyamine uptake capacities of a specific cell line after incubation with fluorescently labeled polyamines and mean fluorescence intensity acquisition via flow cytometry.
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Article
Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction
Review: The development of the Lyso-IP approach and similar methods now allow for lysosomal purification within ten minutes. This review discusses the impact of this new methodology in uncovering the role of lysosomes in neurodegenerative conditions.
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Article
Neuropathological Features of Gaucher Disease and Gaucher Disease with Parkinsonism
Review: To better understand the disease pathogenesis of Gaucher Disease, the authors reviewed the neuropathological features associated with glucocerebrosidase deficiency, examining autopsy studies of rare patients with GD.
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Article
The lipid flippase ATP10B enables cellular lipid uptake under stress conditions
The authors’ data show that the endo-/lysosomal lipid flippase ATP10B contributes to cellular PC uptake under specific cell stress conditions.
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Article
Novel green fluorescent polyamines to analyze ATP13A2 and ATP13A3 activity in the mammalian polyamine transport system
Preprint: Cells acquire the polyamines putrescine (PUT), spermidine (SPD), and spermine (SPM) via the complementary action of polyamine uptake and synthesis pathways. The endosomal P5B-type ATPases ATP13A2 and ATP13A3 emerge as major determinants of mammalian polyamine uptake. Our biochemical evidence shows that fluorescently labeled polyamines are genuine substrates of ATP13A2.
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Article
Rodent models based on endolysosomal genes involved in Parkinson’s disease
Review: This review summarizes parkinsonian phenotypes in rodent models targeting genes that have a role in endolysosomal pathways and future steps to better understand the contribution of endolysosomal dysfunction to PD.
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Article
Inter-organellar communication in Parkinson’s and Alzheimer’s disease: looking beyond endoplasmic reticulum-mitochondria contact sites
Review: Here, authors summarize the contributions of membrane contact sites in dysregulation of inter-organellar communication, taking findings from Parkinson’s and Alzheimer’s as major examples.
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