FISH CODETECTION
By onDIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections
A TIRF microscopy-based assay to monitor LRRK2-dependent endo-lysosome exocytosis
By onHere we describe a method for monitoring CD63+ endo-lysosome exocytosis in hTERT-RPE cells stably expressing CD63-pHluorin.
Whole-cell patch-clamp of PSC-derived cortical glutamatergic and GABAergic neurons
By onThis protocol describes whole-cell-patch-clamp of PSC-derived cortical glutamatergic and GABAergic neurons.
Stereotaxic viral injection surgeries for multifiber photometry recordings and optical stimulation of dopamine neurons
By onThis protocol outlines the surgical procedures for stereotaxic viral injections of genetically encoded sensors and opsins and chronic optical fiber implantation in the mouse striatum for monitoring acetylcholine and dopamine during behavioural tasks.
Multi-fiber photometry in head-fixed mice during spontaneous behavior and dual-cue delay Pavlovian association
By onThis protocol outlines multi-fiber photometry recordings in head-fixed mice during spontaneous behavior, unpredicted reward, and a dual-cue delay Pavlovian task.
Quantification of glutamate release from human iPSC-derived Dopamine Neurons (DaNs)
By onThis protocol describes the procedure to quantify glutamate released from induced pluripotent stem cells derived dopamine neurons (iPSC-DANs) cultured in adherent monolayer.
Whole-cell patch-clamp of human iPSC-derived Dopamine Neurons (DaNs)
By onThis protocol measures electrophysiological properties of individual human dopamine neurons using whole-cell patch clamp.
Cytotoxicity BioAssay of human iPSC-derived Dopamine Neurons (DANs)
By onThis protocol details a sensitive, fast, high-throughput and non-destructive cytolysis assay that measures adenylate kinase (AK) release from damaged cells.
Western Blot of human iPSC-derived Dopamine Neurons (DaNs)
By onThis protocol describes a standard procedure for protein separation and identification of protein of interest.
Validation of RNA oxidation antibody in striatal rat slices
By onValidation of RNA oxidation antibody in striatal rat slices
Staining of RAW cells for RNA oxidation
By onValidation of RNA oxidation antibody in cultured RAW cells
Intracellular Cytokine Staining (ICS) Protocol for FACS Analysis
By onIntracellular staining of lymphocytic cells for Fluorescence-Activated Cell Sorting (FACS) analysis
Open Field Behavior in Mice-Ding Lab
By onThe open field test is widely used to assess spontaneous locomotor activity, exploratory behavior, and anxiety-like responses in rodents.
Single-cell extracellular recordings (surgery, STN optotagging, LHb recordings)
By onProtocol for single cell extracellular recordings (surgery, STN optotagging, LHb recordings)
Electrophysiological Recordings
By onProtocol for electrophysiological patch clamp/voltage clamp recordings.
Protocol for Stasiak et al. 2026 (TREV-Emotion) Manuscript
By onProtocol for Stasiak et al. 2026 (TREV-Emotion) Manuscript. Describes participant preparation, shock calibration, scan sequence, active escape task, and participant debriefing.
Opto-Open Field Test
By onProtocol for optogenetic open field test, which records mouse behavior in an open field arena in response to alternating 5-minute periods of optogenetic stimulation.
