Mouse Stereotaxic Surgery-Arber Lab
By onThis protocol describes the steps for performing stereotaxic surgery in mice. It is applicable to intracranial injections (e.g. virus, drug) and placement of implants (e.g. optical fibers, electrode arrays) into targeted regions of mouse brains.
Optimized Stereotactic Injection Protocol for Targeting the Locus Coeruleus with Minimal Neurotoxicity
By onRefined stereotactic injection protocol targeting the Locus Coeruleus minimizes neurotoxicity.
Stereotaxic virus injection in the SNpc of rats
By onProtocol for stereotaxic virus injection in the substantia nigra pars compacta of rats
6-OHDA mouse model of Parkinson’s disease
By onProtocol for creating a Parkinson's disease mouse model using 6-OHDA involves injecting different doses into the brain to induce varying levels of dopaminergic damage.
Levodopa treatment for low-dose 6-OHDA-treated mice
By onThis protocol describes a one-week levodopa treatment for mice partially depleted of dopamine.
Stereotaxic intracranial virus injection in mice
By onThis protocol outlines steps for conducting stereotaxic brain injections of virus in mice in the Ding lab.
Stereotaxic Optic Fiber Implantation in Mice
By onThis protocol describes implantation of one or multiple optic fiber cannulae in mouse brains used for optical recording brain activity using fiber photometry or for optogenetic manipulation of neuronal activity in mice performing behavioral tasks.
Costa Lab Nanoject Virus Injections
By onThis is the standard stereotaxic/Nanoject injection mouse surgery protocol for the Costa lab at Columbia University.
Chronic Cranial Window and Micro-Optical Probe Implantation in Mice
By onMonitoring neuronal structure and activity in behaving animals helps understand movement control. Surgical procedures like chronic cranial window and micro-optical probe implantation allow optical monitoring of neural circuits.
Tamoxifen Administration in Transgenic Mice-Ding Lab
By onThis protocol describes the procedure for inducing CreER-mediated recombination at loxP sites in transgenic mice through the administration of tamoxifen or 4-hydroxytamoxifen (4-OHT).
Standard Operating Procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates
By onThis protocol details standard operating procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates.
Mouse Behavior – Open Field and T-Maze
By onThis protocol describes two behavioral tasks for mice. The first is the Open Field Test, which is used to asses motor behavior, and the second is the T-Maze, which is used to assess spatial learning.
In Vivo Electrophysiology (Mouse)
By onThis protocol describes the in vivo electrophysiology method for recording neuronal activity in mice.
Accelerated Ovarian Failure AOF mouse model: a model of transitional menopause
By onThe novel model of AOF successfully replicates the human peri- and post-menopause stages, including erratic estrous cycles and hormone fluctuations, followed by undetectable estrogen levels.
Collection of protocols for paper: “Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function”
By onThis is a collection of protocols used in a recent preprint by the Deleidi Lab, Team Schapira. You can access pre-print at https://doi.org/10.21203/rs.3.rs-1521848/v1
Gavage – food supplementation for stalled weight gain in mice
By onFeeding protocol for Ndufs2fl/fl mice,
