brain waves image

Output Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

  results for "Team Kirik"
Sort
  • Output Type
  • Program
  • CRN Team Name
  • Theme

Available ASAP-related hPSC collection from Team Studer

Collection of human pluripotent stem cell lines consisting of isogenic GBA, LRRK2, SNCA series, KI-reporter lines for TOMM20, b-actin, LAMB1, LAMP1, a-synuclein overexpression lines, and other hPSC resources.

Program: Collaborative Research Network
Team:
View Lab Material

Collection of protocols of Team Deleidi used in the publication: “”LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease””

Collection of protocols of Team Deleidi used in the publication: ""LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease""

Program: Collaborative Research Network
View Protocol

Collection of protocols for paper: “Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function”

This is a collection of protocols used in a recent preprint by the Deleidi Lab, Team Schapira. You can access pre-print at https://doi.org/10.21203/rs.3.rs-1521848/v1

Program: Collaborative Research Network
View Protocol

Evaluating the performance of polygenic risk profiling across diverse ancestry populations in Parkinson’s disease

Objective This study aims to address disparities in risk prediction by evaluating the performance of polygenic risk score (PRS) models using the 90 risk variants across 78 independent loci previously linked to Parkinson’s disease (PD) risk across…

Program: Global Parkinson's Genetics Program
View Article

Multi-ancestry genome-wide meta-analysis in Parkinson’s disease

Although over 90 independent risk variants have been identified for Parkinson’s disease using genome-wide association studies, all studies have been performed in just one population at the time. Here we performed the first large-scale…

Program: Global Parkinson's Genetics Program
View Article

Human Postmortem-Derived Brain Sequencing Collection (Harmonized Collection)

The Human Postmortem-derived Brain Sequencing Collection is a harmonized repository comprised of sequencing data contributed by ASAP CRN teams.

Program: Collaborative Research Network
View Dataset

Transgenic A53T mice have astrocytic a-synuclein aggregates in dopamine and striatal regions

Using fluorescent immunohistochemistry, astrocyte subtypes were identified and aSyn burden quantified.

Program: Collaborative Research Network
Team:
View Article

Dopamine and cortical iPSC-derived neurons with different Parkinsonian mutations show variation in lysosomal and mitochondrial dysfunction: implications for protein deposition versus selective cell loss

Comparison of dopamine neurons identified lysosomal and mitochondrial dysfunction, as well as increased tau deposition, alpha-synuclein phosphorylation and decreased glucocerebrosidase activity.

Program: Collaborative Research Network
Team:
View Article

Generation of human-induced pluripotent-stem-cell-derived cortical neurons for high-throughput imaging of neurite morphology and neuron maturation

A STAR protocol that describes how to differentiate cryopreserved human cortical neuronal progenitors into mature cortical neurons for high-throughput imaging analysis

Program: Collaborative Research Network
Team:
View Article

Generation of the iPSC line FINi002-A from a male Parkinson’s disease patient carrying compound heterozygous mutations in the PRKN gene

Generation of the iPSC line FINi002-A from a male Parkinson’s disease patient carrying compound heterozygous mutations in the PRKN gene. Primary fibroblasts from a skin biopsy of a male PD patient were reprogrammed using transiently present…

Program: Collaborative Research Network
Team:
View Article

Scripts for snRNAseq data analysis

Scripts for analyzing single nuclei sequencing data from healthy and Parkinson's Disease brains include creating a reference database with transposable element annotations and a file for Cell Ranger to produce snRNA count matrices.

Program: Collaborative Research Network
View Code

Mouse α-synuclein fibrils are structurally and functionally distinct from human fibrils associated with Lewy body diseases

Mouse α-synuclein fibrils have been studied to understand Parkinson's disease and multiple system atrophy. They differ from human fibrils in structure and behavior, impacting research on related diseases and potential treatments.

Program: Collaborative Research Network
Team:
View Article

3D bioprinting of human neural tissues with functional connectivity

Team Scherzer developed a 3D bioprinting platform to assemble tissues with defined human neural cell types in a desired dimension using a commercial bioprinter.

Program: Collaborative Research Network
View Article

Regional Mouse Brain Analysis (Modified QUINT)

This is series of protocols that has been adapted from published and unpublished protocols broadly referred to as the QUINT workflow: QuPath visualization/segmentation QuickNII Brain Atlas Registration QMask Hemispheric Separation Visualign…

Program: Collaborative Research Network
View Protocol

AAV Purification Protocol with Iodixanol gradient

Protocol used in the Kaplitt and Marongiu labs to purify AAVs.

Program: Collaborative Research Network
Team:
View Protocol