A CellProfiler Pipeline for Quantification of p-SNCA in Mouse Striatal Cholinergic and Medium Spiny Neurons
By onBrain sections stained for pSNCA, DARPP-32, ChAT, and DAPI. Z-stacks processed into MIPs for analysis. Outputs include pSNCA intensity and neuronal mask area, normalised for cell type-specific burden using R script.
Mito-IP: Immunopurification of Mitochondria from MitoTag Cultured Cells
By onA novel method called 'Mito-IP' enables rapid and pure mitochondrial isolation in 10 minutes, supporting various analyses. This technique is being extended to isolate other organelles like lysosomes, Golgi, and peroxisomes using epitope tags.
A CellProfiler computational pipeline to quantify localization of PPM1H on mitochondria
By onCellProfiler software pipeline quantifies PPM1H localization on fragmented mitochondria by hypotonic swelling, highlighting membrane contacts. Wild-type MEFs expressing PPM1H-mApple and GFP-Mito are imaged after treatment with oligomycin/antimycin.
Isolation of brain eGFP-cholinergic interneurons by dissociation and flow cytometry
By onProtocol isolates GFP-expressing cholinergic interneurons from mouse dorsal striatum tissue using a method by Lynette Foo (2013).
Quantification of Axonal Projections from Whole-Brain Slide Images
By onQuantification of Axonal Projections from Whole-Brain Slide Images provides a standardized method for processing and analyzing immunofluorescence images of mouse brain sections to quantify axonal projections.
Immunocytochemistry (ICC) detection of key microglia markers in iPSC derived human microglia cells
By onImmunocytochemistry (ICC) detection of key microglia markers in iPSCderived human microglia cells (hMG)
Multicolor fluorescence in situ hybridization and analysis
By onMulticolor fluorescence in situ hybridization and analysis related to LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
LC-MS/MS Analysis
By onLC-MS/MS Analysis related to LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
Lipidomic analysis in HeLa cells
By onA protocol for the lipidomic analysis in HeLa cells is described.
Lipidomic analysis in HeLa cells for HexCer levels
By onA protocol for the lipidomics analysis in HeLa cells for HexCer levels is described.
Immunofluorescence and confocal imaging in brain sections and image analysis
By onImmunofluorescence and confocal imaging in brain sections and image analysis for Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
SIM imaging and analysis for active zone release sites in vulnerable dopamine axons
By onStereotactic injections and viruses described and SIM imaging and analysis
Striatal synaptosome preparation for imaging
By onStriatal synaptosome imaging and analysis protocols in Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Analysis of ER Flux in Cultured Induced Neurons using Keima ER reporters
By onAssociated with preprint: https://doi.org/10.1101/2023.06.26.546565
Characterizing spatial and temporal properties of ER-phagy receptors
By onAssociated with preprint: https://doi.org/10.1101/2023.06.26.546565
Analysis of ER structures in Cultured Induced Neuron axons
By onAssociated with preprint: https://doi.org/10.1101/2023.06.26.546565
Single cell/nuclei RNAseq analysis
By onThis protocol describes the process for the single cell/nuclei RNA sequencing data of the manuscript "L1retrotransposons drive human neuronal transcriptome complexity and functional diversification " from fetal forebrain and adult prefrontal cortex tissue.
CD8 Cell Density In Substantia Nigra And Cerebral Peduncle Image Analysi
By onQuPath is a bioimage analysis software designed for digital pathology and whole slide image analysis. This protocol describes how to measure CD8 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.
