Metabolomic analysis of polyamines
By onProtocol outlines metabolite extraction, derivatization, and mass spectrometry for various sample types. It covers sample preparation, LC-MS parameters, and data analysis for polyamine profiling.
Bulk RNA-seq Protocol for Astrocytes
By onProtocol for RNA extraction from astrocytes for bulk RNA sequencing to analyze gene expression profiles.
SAM ELISA
By onProtocol for preparing cell lysates and conducting ELISA to quantify proteins is outlined.
CXCL1 ELISA
By onProtocol for ELISA includes sample prep & assay to quantify antigens in biological samples.
Protocol for Live-Cell Imaging of Astrocytes Exposed to HiLyte™ 488-labeled Human Recombinant α-Synucleins
By onProtocol studies impact of HiLyte™ 488-labeled human recombinant α-synuclein on astrocytes through live-cell imaging at different time points post-exposure.
Evaluation of the Inflammatory Secretome of Astrocytes Using Cytokine Array
By onAstrocyte secretome studied with Proteome Profiler™ Human XL Cytokine Array Kit to analyze and quantify cytokine expression levels in conditioned media.
Characterizing spatial and temporal properties of ER-phagy receptors
By onAssociated with preprint: https://doi.org/10.1101/2023.06.26.546565
Analysis of ER structures in Cultured Induced Neuron axons
By onAssociated with preprint: https://doi.org/10.1101/2023.06.26.546565
Analysis of ER Flux in Cultured Induced Neurons using Keima ER reporters
By onAssociated with preprint: https://doi.org/10.1101/2023.06.26.546565
Single cell/nuclei RNAseq analysis
By onThis protocol describes the process for the single cell/nuclei RNA sequencing data of the manuscript "L1retrotransposons drive human neuronal transcriptome complexity and functional diversification " from fetal forebrain and adult prefrontal cortex tissue.
CD8 Cell Density In Substantia Nigra And Cerebral Peduncle Image Analysi
By onQuPath is a bioimage analysis software designed for digital pathology and whole slide image analysis. This protocol describes how to measure CD8 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.
Staining of cells with GolgiTracker for Golgi flow cytometry analysis
By onMethod that allows the staining of intact Golgi using GolgiTracker for subsequent flow cytometry analysis.
Pole Test
By onThe protocol describes the pole test for assessing motor coordination and balance in rodents.
Image processing to investigate NEMO recruitment and involvement in mitophagy and inflammatory signaling
By onExtensive image processing techniques were used to analyze cellular mechanisms, including deconvolution, machine learning, and manual identification of structures. Creative approaches were employed to ensure reliable and reproducible results.
Lipidomic analysis of tissue culture cells, tissues, and purified organelles
By onLipids are a class of molecules that have roles in energy storage, plasma membrane integrity, and signaling events. To gain more understanding of the functions and roles that lipids play in biology, researchers employ discovery analytical approaches, such as mass spectrometry (MS)-based lipidomics. The main objective of this protocol is to provide directive on how to extract lipids from plasma, cells, tissue, and purified organelles for analysis by liquid chromatography (LC)-MS. This analysis will typically yield quantitative data for more than 200 lipids, depending on the sample type analyzed, across a range of lipid classes: phospholipids, cardiolipins, sphingolipids, di- and triacylglyerols, and cholesterylesters
Protein interaction network analysis for Mendelian diseases
By onThis protocol describes the steps to use experimentally validated human data to create a protein-protein interaction network (PPIN) based on disease causative genes. Network analysis (combination of topological functional analyses) will lead to the identification of biological processes relevant for disease and disease endophenotypes.
Confocal imaging and digital image analysis
By onProtocol for confocal imaging on Nikon A1R microscope & digital image analysis is detailed.
