Fluorescently labeled polyamine uptake (via Flow Cytometry)
By onAssess polyamine uptake capacities of a specific cell line after incubation with fluorescently labeled polyamines and mean fluorescence intensity acquisition via flow cytometry.
Immunohistochemistry of rTg4510 mouse brain
By onThe AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds.
Microscopy-based bead protein-protein interaction assay.
By onProtocol for microscopy-based bead protein-protein interaction assay using GST- or mCherry-tagged baits and fluorescently-tagged preys. Requires purified proteins, monitors interactions in equilibrium, and allows quantification of fluorescent signal.
Characterization of Splay Characteristics of Carbon Fiber Electrode Threads (CFETs) in Agar Brain Phantom
By onMethods to measure deflection characteristics of carbon fiber electrodes in agar brain phantoms.
In Vitro FSCV Testing of Carbon Fiber Electrodes to Characterize Functional Operation in Dopamine Detection
By onMethods to measure the performance characteristics of carbon fiber electrodes for neurochemical recording.
Co-immunoprecipitation protocol to study LRRK2 binding to Rab12 in a cell-based assay
By onThe immunoprecipitation of FLAG-tagged LRRK2 from whole cell lysates to assess its interaction with Rab12.
Complex I activity assay
By onThis protocol is part of a Collection of protocols (dx.doi.org/10.17504/protocols.io.8epv593dng1b/v1) for the paper "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function" (https://doi.org/10.21203/rs.3.rs-1521848/v1)
Lentivirus production for primary neuron transduction
By onThis protocol is associated with the following preprint, published on February 19th 2022: The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds Itika Saha, Patricia Yuste-Checa, Miguel Da Silva Padilha, Qiang Guo, Roman Körner, Hauke Holthusen, Victoria A. Trinkaus, Irina Dudanova, Rubén Fernández-Busnadiego, Wolfgang Baumeister, David W. Sanders, Saurabh Gautam, Marc I. Diamond, F. Ulrich Hartl, Mark S. Hipp bioRxiv 2022.02.18.481043; doi: https://doi.org/10.1101/2022.02.18.481043
DQ-BSA assay
By onThis protocol describes the DQ BSA assay for measuring the lysosomal proteolytic activity.
Live-imaging of axonal cargoes in human iPSC-derived neurons or mouse primary neurons
By onThe authors describe the procedure and equipment used for live imaging of axonal cargoes.
GI Transit Assay
By onThis test is used to check for possible signs of constipation and alteration in gut transit.
Behavioral Testing – Open Field and Dyskinesia Scoring
By onThis protocol describes behavioral testing to assess motor deficits in mice. It includes open field locomotor testing and scoring of levodopa-induced dyskinesia.
Investigation_of_mitophagy_in_Hippo_neurons
By onWe developed a method for assessing mitochondrial clearance in primary hippocampal neurons.
HyDrop-RNA v1.0
By onStep-by-step protocol for performing HyDrop-RNA. The duration of each step assumes an experienced protocol user. For a first-time user, we recommend doubling the expected time for each step.
Essential surgeries for the electrophysiological recording from a behaving non-human primate brain 1 (Head fixation post and recording chamber implantation)
By onProtocol for implanting head fixation post and recording chamber in non-human primates for electrophysiological recording.
Halo assay to assess mitophagy
By onThis protocol describes how to assess mitophagy using Halo assay developed by Mizushima lab (DOI: 10.7554/eLife.78923).
Quantification of Extracellular ?-synuclein Assay
By onThis protocol uses a classical ELISA experimental design with sensitive electrochemical detection to provide measure of ?-synuclein concentration in the conditioned media of cultured iPSC-derived human cells.
