Stereotaxic virus injection and fiber optic probe implantation
By onThis protocol serves to prepare mice for in vivo optogenetic stimulation.
Opto-Avoidance-EPM Test
By onThe Opto-Avoidance-Elevated Plus Maze test assesses the effect induced by photostimulation compared to a mouse's natural aversion to open areas.
Opto-RT-CPP Test
By onProtocol for Opto-RT-CPP (Optogenetic Real-Time Conditioned Place Preference) test. This test measures location preference or avoidance in response to optogenetic stimulation.
Opto-Negative Reinforcement Paradigm (Opto-NR)
By onProtocol for Opto-Negative Reinforcement paradigm (Opto-NR). This test uses an operant conditioning box with two nose-poke devices to test aversive learning in mice.
Sugar Positive Reinforcement Paradigm (Sugar-PR)
By onProtocol for Sugar Positive Reinforcement Paradigm (Sugar-PR) with optogenetic stimulation.
Post-injection Histological Analysis
By onProtocol for post-injection histological analysis following optogenetic behavioral assays.
Protocols for “STING dampens the unfolded protein response to enable the presentation of self-antigens on MHC-I during inflammation”
By onSTING pathway regulates MitAP through the UPR, establishing it as a key immune regulator in autoimmunity and PD.
Rotarod with Photometry
By onThis protocol describes collecting in vivo fiber photometry data during rotarod behavior
Ex Vivo Mouse Brain Slice Whole-Cell Patch-Clamp Electrophysiology – Lerner Lab
By onThis protocol outlines steps for performing ex vivo slice whole-cell patch-clamp electrophysiology experiments, including optogenetic stimulation introduced through viral injection or transgenic expression.
Pavlovian protocol – Fushiki et al 2024
By onPavlovian Conditioning Protocols for Mouse Behavioral Studies Derived from Fushiki et al 2024.
Culturing, transfection and NBD-labeled lipid uptake assay in HeLa-CDC50A-OE cells
By onAssay for NBD-labeled lipid uptake in HeLa-CDC50A-OE cells includes seeding, transfection, and flow cytometry to measure flippase construct activity, enabling quantitative assessment of lipid uptake.
all protocols related to “Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency”
By onAll protocols related to "Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency"
Active Escape Protocol (Stasiak et al., 2026)
By onProtocol for Active Escape task by Stasiak et al., referenced in "Integrated Representations of Threat and Control in the Lateral Frontal Pole" (2026) manuscript.
in vivo TurboID
By onProtocol uses iBioID with Lamp1-TurboID in astrocytes to study endo-/lysosomal protein interactions in mice. AAV delivery allows selective biotinylation for proteome mapping, offering insights into astrocyte signaling in vivo.
Protein quantification by SDS-PAGE and Coomassie staining
By onThis protocol provides a step-by-step guide for quantifying protein concentration by SDS-PAGE and Coomassie staining.
Immunoblotting protocol
By onThis protocol outlines a step-by-step method for performing western blot analysis, including protein separation by gel electrophoresis, transfer to membranes, antibody incubation, and detection.
Measurement of ATP13A4 ATPase activity using ADP-Glo Max Assay
By onThis protocol describes a luminescence-based ADP-Glo Max assay (Promega) to measure the ATPase activity of ATP13A4 by detecting the rate of ATP hydrolysis.
Fluorescently labeled polyamine uptake (flow cytometry)
By onAssess polyamine uptake capacities of a specific cell line after incubation with fluorescently labeled polyamines and mean fluorescence intensitiy acquisition via flow cytometry.
Auto-(de)phosphorylation assay
By onProtocol measures ATP13A4 phospho-enzyme levels by detecting its phosphorylated intermediate on an aspartate residue. Utilizes SDS-PAGE under acidic conditions and autoradiography to detect -labeled intermediates.
RT-qPCR (cell lines)
By onThis protocol describes the use of RT-qPCR to assess msAtp13a4 mRNA expression following lentiviral knockdown in C8-D1A cells.
